POLA1 antibody (AA 1406-1439)

Catalog No. ABIN5646961

Product Details anti-POLA1 Antibody

Target: POLA1 (DNA Polymerase alpha (POLA1))

Binding Specificity: AA 1406-1439

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLA1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Expected species reactivity: Human

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1406-1439 from human POLA1 was used as the immunogen for the DNA Polymerase alpha antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the DNA Polymerase alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. WB: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the DNA Polymerase alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Alternative Name: DNA Polymerase alpha / POLA1 (POLA1 Products)

Synonyms: pola antibody, AW321876 antibody, Pola antibody, POLA antibody, p180 antibody, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antibody, polymerase (DNA directed), alpha 1 antibody, DNA polymerase alpha 1, catalytic subunit antibody, pola1.S antibody, Pola1 antibody, POLA1 antibody

Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interactome