SMAD4 antibody (AA 200-300) (Atto 488)

Catalog No. ABIN5651209

Product Details anti-SMAD4 Antibody (Atto 488)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Binding Specificity: AA 200-300

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Detects 60 kDa.

Cross-Reactivity: Human

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide of Human SMAD4 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5651209 was sufficient for detection of SMAD4 in 15 μg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Synonyms: smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody

Background: Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2, Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.

Gene ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy