Recombinant EPH Receptor A2 antibody (AA 183-510)

Catalog No. ABIN5668239

Product Details anti-EPH Receptor A2 Antibody

Target: EPH Receptor A2 (EPHA2)

Antibody Type: Recombinant Antibody

Binding Specificity: AA 183-510

Reactivity: Human

Host: Human

Clonality: Monoclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon), Internalization Assay (INA)

Specificity: 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM, Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.

Characteristics:

OriginalSpeciesName: Human

OriginalFormat: IgG1

Purification: Purified antibody.

Immunogen: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.

Clone: 1C1

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: Ephrin Type A receptor 2 (EPHA2 Products)

Synonyms: ARCC2 antibody, CTPA antibody, CTPP1 antibody, CTRCT6 antibody, ECK antibody, epha2 antibody, MGC89505 antibody, AW545284 antibody, Eck antibody, Myk2 antibody, Sek-2 antibody, Sek2 antibody, id:ibd5152 antibody, rtk6 antibody, G42 antibody, XE10 antibody, epha2-A antibody, EPH receptor A2 antibody, Eph receptor A2 antibody, epoxide hydrolase antibody, eph receptor A2 a antibody, EPH receptor A2 L homeolog antibody, EPHA2 antibody, Epha2 antibody, epha2 antibody, ephA2 antibody, epha2a antibody, epha2.L antibody

Background: EphA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK

UniProt: P29317

Pathways: RTK Signaling