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Recombinant His Tag antibody

WB, ELISA Host: Mouse Monoclonal 3D5 unconjugated Recombinant Antibody

Catalog No. ABIN5668265

Datasheet as PDF

Target See all His Tag Antibodies

His Tag

Antibody Type

Recombinant Antibody

Reactivity

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Host
90

54

12

9

8

7

2

1
Mouse
Compatible Secondaries
Clonality
101

69

10
Monoclonal
Conjugate
87

22

14

11

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
Application
148

99

35

26

24

15

14

14

13

13

12

7

6

6

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA

Specificity

This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

Characteristics

OriginalSpeciesName: Mouse
OriginalFormat: IgG2b

Purification

Purified antibody.

Immunogen

Purified his tagged Fab fragment.

Clone

3D5

Isotype

IgG3 kappa

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anti-His Tag antibody (Biotin)
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Application Notes

Optimal working dilution should be determined by the investigator.

Comment

This is a reformatted mouse IgG3 antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

Restrictions

For Research Use only
Buffer

PBS with 0.02 % Proclin 300.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Target

His Tag

Abstract

His Tag Products

Target Type

Tag

Background

Polyhistidine tag