PEDF antibody (AA 101-200)

Catalog No. ABIN5675472

Product Details anti-PEDF Antibody

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Binding Specificity: AA 101-200

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEDF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PEDF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PEDF

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Alternative Name: PEDF (SERPINF1 Products)

Synonyms: EPC-1 antibody, OI12 antibody, OI6 antibody, PEDF antibody, MGC82984 antibody, SERPINF1 antibody, AI195227 antibody, Pedf antibody, Pedfl antibody, Sdf3 antibody, Dmrs91 antibody, zgc:92072 antibody, serpin family F member 1 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 L homeolog antibody, serine (or cysteine) peptidase inhibitor, clade F, member 1 antibody, SERPINF1 antibody, serpinf1 antibody, serpinf1.L antibody, Serpinf1 antibody

Background:

Synonyms: pigment epithelium-derived factor, Serpin-F1, Stromal cell-derived factor 3, SDF-3, Caspin, Alpha 2 antiplasmin, EPC 1, EPC1, PIG 35, PIG35, Pigment epithelium derived factor, Proliferation inducing protein 35, SERPIN F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, SERPINF1, PEDF_HUMAN

Background: Pigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.

Gene ID: 5176

UniProt: P36955