Collagen IV antibody

Catalog No. ABIN5680246

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Specificity: Human collagen type IV 100 % human collagen types I, II, III, and V <0.1 % human fibronectin and laminin < 0.1 % No cross-reactivity with bovine

No Cross-Reactivity: Cow, Mouse, Pig, Rat

Cross-Reactivity (Details): Human, no cross-reactivity with bovine, mouse, rat and porcine collagens type IV

Characteristics: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100 % , human collagen types I, II, III, and V <0.1 %, human fibronectin and laminin < 0.1 % (RIA at 1:10000 dilution).

Purification: Protein G affinity purified antibody prod. in nude mice.

Immunogen: Purified collagen type IV from human placenta

Clone: 24D1

Isotype: IgG1

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:320, IHC(P) ? 1:4000, ELISA ? 1:4000 (OD ? 500)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: phosphate buffered solution, no BSA and preservative added!

Preservative: Without preservative

Storage: -20 °C

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody