Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody is expected to recognize both reported isoforms (NP_003783.1, NP_694880.1).
Purification
EDF1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
EDF1 Antibody was raised against synthetic peptide NKQHSITKNTAKLDR found at an internal region of EDF1.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 17 kDa band observed in lysates of cell line A549 (calculated MW of 16.4 kDa according to NP_003783.1). Recommended concentration: 0.3-1μ,g/mL. Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
EDF1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
EDF1
(Endothelial Differentiation Related Factor 1 (EDF1))