EEF1A2 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
EEF1A2 Antibody was raised against synthetic peptide NPATVPFVPIS found at an internal region of EEF1A2.
EEF1A2
Reactivity: Human
WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 50 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 50.5 kDa according to NP_001949.1). The same band is observed in lysates of Mouse and Rat Heart and Skeletal Muscle. Recommended concentration: 0.5-2μ,g/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
EEF1A2 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.