MAPKAP Kinase 5 antibody (Internal Region)

Catalog No. ABIN5687996

Product Details anti-MAPKAP Kinase 5 Antibody

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPKAP Kinase 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Purification: MK5 / MAPKAPK5 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: MK5 / MAPKAPK5 Antibody was raised against synthetic peptide PQVLEAQRRHQKEKS found at an internal region of MK5 / MAPKAPK5.

Application Details

Application Notes: ELISA: antibody detection limit dilution 1:64000. Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Bioche Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8μ,g/mL. Immunoprecipitation:MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11927 or EB11928. Western blots of

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: MK5 / MAPKAPK5 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Minimize freezing and thawing.

Target Details for MAPKAP Kinase 5

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Alternative Name: MK5 / MAPKAPK5 (MAPKAPK5 Products)

Synonyms: MAPKAPK5 antibody, MK5 antibody, PRAK antibody, MAPKAP-K5 antibody, MK-5 antibody, mitogen-activated protein kinase-activated protein kinase 5 antibody, MAP kinase-activated protein kinase 5 antibody, MAPKAPK5 antibody, mapkapk5 antibody, Mapkapk5 antibody

Gene ID: 8550

NCBI Accession: NP_003659, NP_620777

UniProt: Q8IW41

Pathways: MAPK Signaling