NFATC3 antibody (Internal Region)

Catalog No. ABIN5688024

Product Details anti-NFATC3 Antibody

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NFATC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is expected to recognize all four reported isoforms (NP_775188.1, NP_004546.1, NP_775186.1, NP_775187.1).

Purification: NFATC3 / NFAT4 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NFATC3 / NFAT4 Antibody was raised against synthetic peptide HQELDAHEDDLQIN found at an internal region (near the N Terminus) of NFATC3 / NFAT4.

Application Details

Application Notes: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 150  kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 116  kDa according to NP_775188.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Re

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: NFATC3 / NFAT4 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Minimize freezing and thawing.

Target Details for NFATC3

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Alternative Name: NFATC3 / NFAT4 (NFATC3 Products)

Synonyms: NFATC3 antibody, NFATx antibody, nf-at antibody, nfat4 antibody, nfatx antibody, C80703 antibody, D8Ertd281e antibody, NFAT4 antibody, NFATX antibody, nuclear factor of activated T-cells 3 antibody, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody, nuclear factor of activated T-cells 3 S homeolog antibody, NFATC3 antibody, nfatc3 antibody, Nfatc3 antibody, nfatc3.S antibody

Molecular Weight: Approx 150kDa

Gene ID: 4775

NCBI Accession: NP_775188, NP_004546, NP_775186, NP_775187

UniProt: Q12968

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Chromatin Binding