Transcription factor E2F1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Transcription factor E2F1 Antibody was raised against synthetic peptide QEVTSEEENRATDS found at an internal region of Transcription factor E2F1.
E2F1
Reactivity: Human
WB, ELISA, IF (cc), IF (p), FACS, IHC (p), ICC, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 46.9 kDa according to NP_005216.1). Recommended concentration: 1-3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Transcription factor E2F1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.