Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
Recognizes human progranulin. Detects a band of ~90 kDa by Western blot. Weakly cross-reacts with mouse progranulin. Cross-reacts with hGRN-C and hGRN-F.
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections, 10 μg/mL). Western Blot: (1:2,000-1:5,000 using ECL, suggested blocking and dilution buffer is PBST containing 0.05 % Tween 20 and 5 % skim milk, suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Stable for at least 6 months after receipt when stored at -20°C.
Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer's Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.