ALOX5 antibody (pSer523)

Catalog No. ABIN5688904

Product Details anti-ALOX5 Antibody

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Binding Specificity: pSer523

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Affinity Purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-lipoxygenase (5-LO).

Application Details

Application Notes: The antibody has been directly tested for reactivity in Western blots with rat and human tissue. It is anticipated that the antibody will react with non human primates based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: 5 Lipoxygenase antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: 5 Lipoxygenase (ALOX5 Products)

Synonyms: 5-LO antibody, 5-LOX antibody, 5LPG antibody, LOG5 antibody, 5LO antibody, 5LX antibody, AI850497 antibody, F730011J02 antibody, LOX5A antibody, si:dkey-194n13.2 antibody, lox5 antibody, arachidonate 5-lipoxygenase antibody, arachidonate 5-lipoxygenase a antibody, Arachidonate 5-lipoxygenase antibody, ALOX5 antibody, Alox5 antibody, alox5a antibody, AFUA_4G02770 antibody, AOR_1_1362014 antibody, lox5 antibody

Background: The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

Molecular Weight: 80 kDa

Gene ID: 240

UniProt: P09917