FGF2 antibody (Biotin)
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- Target See all FGF2 Antibodies
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FGF2 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Purification
- Anti-hFGF-basic specific antibody was purified by affinity chromatography and then biotinylated.
- Immunogen
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-basic (human FGF-basic).
- Top Product
- Discover our top product FGF2 Primary Antibody
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- Application Notes
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ELISA:
To detect hFGF-basic by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFGF-basic.
Sandwich: To detect hFGF-basic by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-basic (XP-5137) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic.
Western Blot:
To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Storage
- -20 °C
- Storage Comment
- FGF-basic antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Target
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- Alternative Name
- FGF-basic (FGF2 Products)
- Synonyms
- BFGF antibody, FGF-2 antibody, FGFB antibody, HBGF-2 antibody, Fgf-2 antibody, Fgfb antibody, bFGF antibody, fibroblast growth factor 2 antibody, fibroblast growth factor 2 (basic) antibody, FGF2 antibody, Fgf2 antibody, fgf2 antibody
- Gene ID
- 2247
- UniProt
- P09038
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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