GDNF antibody

Catalog No. ABIN5689004

Product Details anti-GDNF Antibody

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDNF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Anti-hGDNF specific antibody was purified by affinity chromatography employing immobilized hGDNF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDNF (human Glial-Derived Neurotrophic Factor).

Application Details

Application Notes: ELISA:

To detect hGDNF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGDNF.

Sandwich: To detect hGDNF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GDNF (XP-5143Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDNF.

Western Blot:

To detect hGDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: GDNF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF (GDNF Products)

Synonyms: gdnf antibody, GDNF antibody, AI385739 antibody, gdnfa antibody, hfb1-gdnf antibody, ATF1 antibody, ATF2 antibody, HFB1-GDNF antibody, HSCR3 antibody, glial cell derived neurotrophic factor antibody, glial cell derived neurotrophic factor a antibody, glial cell line derived neurotrophic factor antibody, glial cell derived neurotrophic factor L homeolog antibody, Gdnf antibody, gdnfa antibody, GDNF antibody, gdnf.L antibody

Gene ID: 2668

UniProt: P39905

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration