PAK1/2/3 antibody

Catalog No. ABIN5689497

Product Details anti-PAK1/2/3 Antibody

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous level of total PAK1/PAK2/PAK3 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: PAK1/PAK2/PAK3 (Ab-423/402/421) antibody was raised against a peptide sequence around aa.421~425/400~404/419~423 (R-S-T-M-V) derived from Human PAK1/PAK2/PAK3.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK1 / PAK2 / PAK3 (PAK1/2/3 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, pak1.S antibody, PAK1 antibody

Background: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Molecular Weight: 61 - 67 (PAK2) 68 - 74 (PAK1/3) kDa

Gene ID: 5058, 5062, 5063

NCBI Accession: NP_001122092, NP_002568, NP_001121638

UniProt: Q13153, Q13177, O75914