IKK alpha antibody (AA 21-25)

Catalog No. ABIN5689587

This Rabbit Polyclonal antibody specifically detects IKK alpha in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IKK alpha antibody (AA 21-25) (ABIN5689587)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IKK alpha Antibody

Binding Specificity: AA 21-25

Specificity: This antibody detects endogenous level of total IKKα, protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: IKK a (Ab-23) antibody was raised against a peptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK α,.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: IKK a

Background: Acts as part of the IKK complex in the conventional pathway of NF-κ,-B activation and phosphorylates inhibitors of NF-κ,-B thus leading to the dissociation of the inhibitor/NF-κ,-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ,-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ,-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-κ,-B-regulated promoters during inflammatory responses triggered by cytokines.

Molecular Weight: 85 kDa

Gene ID: 1147

NCBI Accession: NP_001269

UniProt: O15111

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins