IKK alpha antibody (AA 21-25)

Catalog No. ABIN5689587

Product Details anti-IKK alpha Antibody

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Binding Specificity: AA 21-25

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: This antibody detects endogenous level of total IKKα, protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: IKK a (Ab-23) antibody was raised against a peptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK α,.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: IKK a (CHUK Products)

Synonyms: ikk antibody, ikk1 antibody, ikka antibody, ikkalpha antibody, CHUK antibody, IKBKA antibody, IKK-alpha antibody, IKK1 antibody, IKKA antibody, NFKBIKA antibody, TCF16 antibody, AI256658 antibody, Chuk1 antibody, Fbx24 antibody, Fbxo24 antibody, Ikka antibody, zgc:56539 antibody, conserved helix-loop-helix ubiquitous kinase S homeolog antibody, conserved helix-loop-helix ubiquitous kinase antibody, chuk.S antibody, CHUK antibody, Chuk antibody, chuk antibody

Background: Acts as part of the IKK complex in the conventional pathway of NF-κ,-B activation and phosphorylates inhibitors of NF-κ,-B thus leading to the dissociation of the inhibitor/NF-κ,-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ,-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ,-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-κ,-B-regulated promoters during inflammatory responses triggered by cytokines.

Molecular Weight: 85 kDa

Gene ID: 1147

NCBI Accession: NP_001269

UniProt: O15111

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins