Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
This antibody detects endogenous level of AMPKα,1 only when phosphorylated at serine 487.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen
AMPKa1 (Phospho-Ser487) antibody was raised in was raised against a peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKα,1.
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.