Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
This antibody detects endogenous level of ATF-2 only whenphosphorylated at threonine 73 or 55.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen
ATF2 (Phospho-Thr73 or 55) antibody was raised against a peptide sequence around phosphorylation site of threonine 73 or 55 (T-P-T (p) -R-F) derived from Human ATF2.
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.