MEK2 antibody (pThr394)

Catalog No. ABIN5689820

Product Details anti-MEK2 Antibody

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Binding Specificity: pThr394

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: This antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: MEK-2 (Phospho-Thr394) antibody was raised against a peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name: MEK 2 (MAP2K2 Products)

Synonyms: CFC4 antibody, MAPKK2 antibody, MEK2 antibody, MKK2 antibody, PRKMK2 antibody, AA589381 antibody, MK2 antibody, Prkmk2 antibody, ATMKK2 antibody, F27B13.50 antibody, F27B13_50 antibody, MAP KINASE KINASE 1 antibody, MAP kinase kinase 2 antibody, MK1 antibody, map2k2 antibody, wu:fa56c06 antibody, zgc:123171 antibody, zgc:172250 antibody, mitogen-activated protein kinase kinase 2 antibody, mitogen activated protein kinase kinase 2 antibody, MAP kinase kinase 2 antibody, mitogen-activated protein kinase kinase 2a antibody, mitogen-activated protein kinase kinase 2b antibody, MAP2K2 antibody, Map2k2 antibody, MKK2 antibody, map2k2a antibody, map2k2b antibody

Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.

Molecular Weight: 44 kDa

Gene ID: 5605

NCBI Accession: NP_109587

UniProt: P36507

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling