PINK1 antibody (AA 120-170)

Catalog No. ABIN5690101

Product Details anti-PINK1 Antibody

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Binding Specificity: AA 120-170

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PINK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: PINK1 antibody is human, mouse and rat reactive. At least two isoforms are known to exist, this antibody will only detect the longer isoform. PINK1 antibody will detect the cleaved and uncleaved form of PINK1.

Purification: PINK1 antibody is affinity chromatography purified via peptide column.

Immunogen: PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1.

The immunogen is located within amino acids 120 - 170 of PINK1.

Isotype: IgG

Application Details

Application Notes: PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PINK1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: PINK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for PINK1

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Alternative Name: PINK1 (PINK1 Products)

Synonyms: BEST:GH23468 antibody, CG4523 antibody, Dmel\\CG4523 antibody, PINK antibody, PINK1 antibody, dPINK1 antibody, dPink1 antibody, pink1 antibody, wu:fc39e12 antibody, zgc:101729 antibody, BRPK antibody, PARK6 antibody, 1190006F07Rik antibody, AU042772 antibody, AW557854 antibody, mFLJ00387 antibody, PTEN-induced putative kinase 1 antibody, PTEN induced putative kinase 1 antibody, Pink1 antibody, PINK1 antibody, pink1 antibody

Background: The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1). PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2). PINK1 is cleaved by the mitochondrial protease PARL (3). Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4).

Molecular Weight: Predicted: 55 kDa

Observed: 53 kDa

Gene ID: 65018

NCBI Accession: NP_115785

UniProt: Q9BXM7

Pathways: Autophagy