ICAM1 antibody
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- Target See all ICAM1 Antibodies
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ICAM1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Immunogen
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1). Human TIMP-1 specific antibody was purified by affinity chromatography and then biotinylated.
- Top Product
- Discover our top product ICAM1 Primary Antibody
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- Application Notes
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human ICAM-1 (10 ng/mL), a concentration of 8.0 μ,/mL of this antibody is required.
ELISA:
Direct:
To detect hTIMP-1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.
Sandwich
To detect hTIMP-1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human TIMP-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.
Western Blot:
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Storage
- -20 °C
- Storage Comment
- ICAM-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Target
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- Alternative Name
- ICAM-1 (ICAM1 Products)
- Synonyms
- BB2 antibody, CD54 antibody, P3.58 antibody, Icam-1 antibody, Ly-47 antibody, MALA-2 antibody, ICAM antibody, ICAM-1 antibody, ICAM1 antibody, intercellular adhesion molecule 1 antibody, intercellular adhesion molecule-1 antibody, ICAM1 antibody, Icam1 antibody, ICAM-1 antibody
- Target Type
- Viral Protein
- Gene ID
- 3383
- UniProt
- P05362
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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