Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNeuroserpin (human Neuroserpin). Human Neuroserpin specific antibody was purified by affinity chromatography employing immobilized hNeuroserpin matrix.
SERPINI1
Reactivity: Human
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Indirect: To detect hNeuroserpin by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.
Sandwich To detect hNeuroserpin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Neuroserpin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.
Western Blot: To detect hNeuroserpin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeuroserpin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Storage
-20 °C
Storage Comment
Neuroserpin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Target
Neuroserpin (SERPINI1)
(serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))
PI12 antibody, neuroserpin antibody, AI837402 antibody, Ns antibody, PI-12 antibody, Spi17 antibody, CG9453 antibody, Dmel\\CG9453 antibody, Serp2 antibody, Sp4 antibody, Spn4 antibody, Spn4A antibody, dSerp2 antibody, sp4 antibody, spn4 antibody, raPIT5a antibody, SERPINI1 antibody, pi12 antibody, serpini1l antibody, si:ch211-167c22.4 antibody, serpin family I member 1 antibody, serine (or cysteine) peptidase inhibitor, clade I, member 1 antibody, Serpin 42Da antibody, serpin peptidase inhibitor, clade I (neuroserpin), member 1 antibody, serpin family I member 1 L homeolog antibody, SERPINI1 antibody, Serpini1 antibody, Spn42Da antibody, serpini1 antibody, serpini1.L antibody
Background
Neuroserpin is a member of the serpin superfamily and is predominantly expressed by neurons of the developing and adult brain. This serpin is secreted from axonal growth cones of the CNS and PNS where it inhibits the enzyme tissue plasminogen activator. The expression pattern of neuroserpin and its in vivo inhibitory activity implicate this serpin in regulating axonal growth, reducing seizure activity, controlling damage in cerebral infarction and regulating emotional behaviour and memory. Four different mutations have been described in the human neuroserpin gene that promote neuroserpin abnormal polymerization, giving rise to intraneuronal inclusion bodies and a new form of dementia called Familial Encephalopathy with Neuroserpin Inclusion Bodies.