E2F1 antibody (AA 162-476)

Catalog No. ABIN1721511

Product Details anti-E2F1 Antibody

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Binding Specificity: AA 162-476

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Reacts with human E2F-1 (aa 244-264).

Purification: Purified (protein G)

Immunogen: GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.

Clone: 18E10

Isotype: IgG1 kappa

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC(1 - 5 μg/mL) IP(1 - 5 μg/mL) IHC(1 - 5 μg/mL) WB(1 - 5 μg/mL)

Positive control: E2F-1 transfected Soas2 cells and recombinant fusion protein

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4.

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: -80 °C

Storage Comment: This antibody is stable for at least one year at -70°C. Avoid multiple freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F-1 (E2F1 Products)

Synonyms: CG6376 antibody, DRTF1/E2F antibody, DmE2F-1 antibody, Dmel\\CG6376 antibody, Dp antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E(var)3-95E antibody, E(var)93E antibody, E2F antibody, E2F-1 antibody, E2F1 antibody, E2f1 antibody, Evar(3)164 antibody, dE2F antibody, dE2F1 antibody, dE2f antibody, dE2f1 antibody, de2f1 antibody, def21 antibody, drosE2F1 antibody, e2f antibody, e2f1 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, mKIAA4009 antibody, xE2F antibody, ATE2FB antibody, E2F TRANSCRIPTION FACTOR-1 E2F1 antibody, E2F transcription factor 1 antibody, E2FB antibody, E2FB TRANSCRIPTION FACTOR antibody, E2F transcription factor 1 antibody, E2F transcription factor 1 L homeolog antibody, E2f1 antibody, E2F1 antibody, e2f1.L antibody

Background: Transcription factor E2F1 PBR3 Retinoblastoma-associated protein 1, RBAP-1 Retinoblastoma-binding protein 3, RBBP-3 pRB-binding protein E2F-1 Gene name : E2F1

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy