MSH2 antibody
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- Target See all MSH2 Antibodies
- MSH2 (Mismatch Repair Protein 2 (MSH2))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MSH2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)
- Immunogen
- mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
- Isotype
- IgG
- Top Product
- Discover our top product MSH2 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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HeLa cells were subjected to SDS PAGE followed by western blot with FNab05371(MSH2 antibody) at dilution of 1:500
- Restrictions
- For Research Use only
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- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze / thaw cycles.
- Storage
- -20 °C
- Expiry Date
- 12 months
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- Target
- MSH2 (Mismatch Repair Protein 2 (MSH2))
- Alternative Name
- MSH2 (MSH2 Products)
- Synonyms
- COCA1 antibody, FCC1 antibody, HNPCC antibody, HNPCC1 antibody, LCFS2 antibody, AI788990 antibody, wu:fc06b02 antibody, wu:fc13e09 antibody, zgc:55333 antibody, ATMSH2 antibody, MUTS homolog 2 antibody, msh2 antibody, mutS homolog 2 antibody, mutS homolog 2 (E. coli) antibody, MUTS homolog 2 antibody, mutS homolog 2 L homeolog antibody, MutS protein homolog 2 antibody, MSH2 antibody, Msh2 antibody, msh2 antibody, msh2.L antibody
- Background
- Synonyms:COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2 Background:Component of the post-replicative DNA mismatch repair system(MMR). Forms two different heterodimers: MutS alpha(MSH2-MSH6 heterodimer) and MutS beta(MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
- Molecular Weight
- 100 kDa-105 kDa
- Gene ID
- 4436
- UniProt
- P43246
- Pathways
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
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