CDC25B antibody

Catalog No. ABIN5700493

Product Details anti-CDC25B Antibody

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Immunogen: cell division cycle 25 homolog B (S. pombe)

Isotype: IgG

Application Details

Application Notes: WB : 1:200-1:1000 IP :1:200-1:1000 IHC : 1:20-1:200

Comment:

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01522(CDC25B antibody) at dilution of 1:300

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B (CDC25B Products)

Synonyms: CDC25B antibody, AI604853 antibody, cell division cycle 25B antibody, cdc25b antibody, CDC25B antibody, Cdc25b antibody

Background: Synonyms:CDC25B, CDC25HU2, M phase inducer phosphatase 2 Background:CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

Molecular Weight: 60-70 kDa

Gene ID: 994

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy