SMAD4 antibody
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- Target See all SMAD4 Antibodies
- SMAD4 (SMAD Family Member 4 (SMAD4))
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SMAD4 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Immunogen
- SMAD family member 4
- Isotype
- IgG
- Top Product
- Discover our top product SMAD4 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab07995(SMAD4 antibody) at dilution of 1:600
- Restrictions
- For Research Use only
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- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze / thaw cycles.
- Storage
- -20 °C
- Expiry Date
- 12 months
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- Target
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Alternative Name
- SMAD4 (SMAD4 Products)
- Synonyms
- smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody
- Background
- Synonyms:DPC4, MADH4 Background:In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein(BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements(SBEs)(5'-GTCT/AGAC-3') within BMP response element(BMPRE) of cardiac activating regions(By similarity). Common SMAD(co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta(transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site, required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
- Molecular Weight
- 48 kDa
- Gene ID
- 4089
- UniProt
- Q13485
- Pathways
- Cell Division Cycle, Chromatin Binding, Autophagy
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