Leptin antibody

Catalog No. ABIN103522

Product Details anti-Leptin Antibody

Target: Leptin (LEP)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics:

Synonyms: rabbit anti-leptin antibody, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody

Background: Anti-Leptin antibody detects leptin protein. Leptin may function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes. Anti-leptin Antibody is ideal for investigators involved in Cell Signaling, Obesity research, aging research and Signal Transduction research.

Gene Name: Leptin

Purification: Anti-Leptin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  The antibody will recognize leptin from mouse and human.  Reactivity with leptin from other sources is unknown.

Immunogen:

Immunogen: Anti-Leptin antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This IgG fraction of anti-Mouse Leptin was tested for use in ELISA and immunoblotting.  Reactivity in other immunoassays is unknown. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin (LEP Products)

Synonyms: ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody

Gene ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process