MEK1 antibody (C-Term) (FITC)

Catalog No. ABIN5706849

Product Details anti-MEK1 Antibody (FITC)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEK1 antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA

Characteristics:

Synonyms: mouse anti-MEK1 antibody FITC conjugation, fluorescein conjugated mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody

Background: MEK1 antibodies detect the MEK1 isoform.  Mitogen-activated protein kinase kinase 1, also known as MKK or MEK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain.  Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase.   MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain.   The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Gene Name: MAP2K1

Purification: This FITC conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK1. Anti-MEK1 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.

Immunogen:

Immunogen: Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Immunogen Type: Peptide

Clone: 13B6-G12

Isotype: IgG

Labeling Ratio: 3.5

Application Details

Application Notes:

Application Note: Anti-MEK 1 FITC Conjugated (MOUSE) Antibody is suitable for use in Western Blotting and ELISA.  Specific conditions of reactivity should be optimized by the end user.  Expect a band of approximately 43.5 kDa.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1 (MAP2K1 Products)

Synonyms: Mek1 antibody, CFC3 antibody, MAPKK1 antibody, MEK1 antibody, MKK1 antibody, PRKMK1 antibody, MEKK1 antibody, Prkmk1 antibody, mek-2 antibody, si:ch211-242m18.2 antibody, wu:fj56a12 antibody, wu:fj61b01 antibody, zgc:56557 antibody, ATMEK1 antibody, F20B18.180 antibody, F20B18_180 antibody, MAP kinase/ ERK kinase 1 antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 antibody, NMAPKK antibody, MEK antibody, mitogen activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 L homeolog antibody, MAP kinase/ ERK kinase 1 antibody, Map2k1 antibody, MAP2K1 antibody, map2k1.L antibody, map2k1 antibody, MEK1 antibody

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling