PCSK6 antibody (AA 637-648)

Catalog No. ABIN574302

Product Details anti-PCSK6 Antibody

Target: PCSK6 (Proprotein Convertase Subtilisin/kexin Type 6 (PCSK6))

Binding Specificity: AA 637-648

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PCSK6 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PCSK6 / PACE4. This antibody is expected to recognize reported isoforms a, b, g and h (NP_002561.1, NP_612192.1, NP_612193.1, NP_612194.1 resp.).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rabbit (100%) Marmoset (92%) Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Pig, Opossum (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-YHTFSAHQSRSR from an internal region of human PCSK6 / PACE4 (NP_002561.1, NP_612192.1, NP_612195.1, NP_612197.1, NP_612196.1, NP_612198.2, NP_612193.1, NP_612194.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rabbit (100%), Marmoset (92%), Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Pig, Opossum (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 40-45 kD band in Human Brain (Cerebellum) lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106 kD according to NP_002561.1. The 40-45 kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PCSK6

Target: PCSK6 (Proprotein Convertase Subtilisin/kexin Type 6 (PCSK6))

Alternative Name: PACE4 / PCSK6 (PCSK6 Products)

Synonyms: PACE4 antibody, PCSK6 antibody, SPC4 antibody, C86343 antibody, Pace4 antibody, Spc4 antibody, PACE4AIIa antibody, Xpace4 antibody, spc4 antibody, proprotein convertase subtilisin/kexin type 6 antibody, peptidase S8 antibody, proprotein convertase subtilisin/kexin type 6 L homeolog antibody, PCSK6 antibody, EAMY_RS27925 antibody, pcsk6 antibody, Pcsk6 antibody, pcsk6.L antibody

Background: Name/Gene ID: PCSK6
Subfamily: Serine S8
Family: Protease

Synonyms: PCSK6, PACE4, SPC4

Gene ID: 5046

NCBI Accession: NP_002561, NP_612192, NP_612195, NP_612197, NP_612196, NP_612198, NP_612193, NP_612194

UniProt: P29122

Pathways: Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome