CRYAB antibody (C-Term)

Catalog No. ABIN615274

Product Details anti-CRYAB Antibody

Target: CRYAB (Crystallin, alpha B (CRYAB))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody reacts to Alpha Crystallin B Chain (CRYAB).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Porcine, Bovine and Rabbit.
Species reactivity (tested):Human.

Purification: Protein A Chromatography

Immunogen: CRYAB antibody was raised against synthetic peptide derived from sequence near the carboxy-terminus of human alphaB-Crystallin conjugated to KLH .Remarks: Sequence is identical in rat, rabbit, and bovine.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS containing 0.09 % sodium azide as preservative and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: alpha-Crystallin B Chain (CRYAB Products)

Synonyms: CMD1II antibody, CRYA2 antibody, CTPP2 antibody, CTRCT16 antibody, HSPB5 antibody, MFM2 antibody, Crya-2 antibody, Crya2 antibody, HspB5 antibody, AACRYA antibody, CRYAB antibody, cryab antibody, cryab2 antibody, wu:fe37f08 antibody, zgc:91937 antibody, crystallin alpha B antibody, crystallin, alpha B antibody, hypothetical protein antibody, crystallin, alpha B, a antibody, crystallin, alpha B, b antibody, CRYAB antibody, Cryab antibody, ZK1128.7 antibody, cryaba antibody, cryabb antibody

Background: Alpha-crystallins composed of ~20 kDa alphaA and alphaB subunits function as major water-soluble proteins accounting for almost 50 % of total protein in the mammalian transparent eye lens, also existing in a variety of other tissues1. Crystallin families beta and gamma share homology with each other but not the alpha-crystallin family or the small heat shock protein (sHsp) family. sHsps including the alpha- crystallin proteins are induced by heat and other stress insults in a variety of organisms. The alpha-crystallins possess structural and functional similarities and share sequence homology with Hsp25/273. Most sHsps exhibit four common structural and functional features: monomeric molecular weight between 12 and 43 kDa, the formation of large oligomeric complexes especially for alphaA-crystallin, alphaB-crystallin and Hsp25/27, a moderately conserved alpha-crystallin domain in the central region of the protein, and molecular chaperone activity. The alpha-crystallin domain bounded by variable N-terminal and C-terminal extensions contains approximately 80 residues and participates in oligomer assembly. Oligomers, potentially 800 kDa or more, exhibit dynamic subunit exchanges and organizational plasticity, which may promote functional diversity. Phosphorylation of serine residues specifically for Hsp27 occurs in response to stress during development, typically decreasing oligomer size. Chaperone activity requires oligomerization (which, in turn, modulates the chaperone activity) and is confined to binding unfolded intermediates to prevent irreversible aggregation, even though productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization and modulation of intermediate filament organization during physiological stress and neurodegenerative disease.Synonyms: Alpha(B)-crystallin, CRYA2, CRYAB, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Gene ID: 1410

NCBI Accession: NP_001876

UniProt: P02511