TSHR, B Chain antibody (Biotin)

Catalog No. ABIN6154243

Product Details anti-TSHR, B Chain Antibody (Biotin)

Target: TSHR, B Chain (Thyroid Stimulating Hormone Receptor, B Chain (TSHR, B Chain))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TSHR, B Chain antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: Mouse Monoclonal anti-TSH Receptor, B chain (TSHRB/1405), Biotin Conjugate

Characteristics: Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Grave s disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: B-Chain protein|Recombinant human TSHR

Clone: TSHRB-1405

Isotype: IgG2a

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA  pH 8.0 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T Cells. Thyroid gland

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for TSHR, B Chain

Target: TSHR, B Chain (Thyroid Stimulating Hormone Receptor, B Chain (TSHR, B Chain))

Alternative Name: TSH Receptor, B chain (TSHR, B Chain Products)

Background: CHNG1, hTSHRI, LGR3, Thyroid-stimulating hormone receptor, Thyrotropin receptor, Thyrotropin receptor I, TSHR

Molecular Weight: 42 kDa

Gene ID: 7253, 160411

UniProt: P16473