Cyclin A antibody (Biotin)

Catalog No. ABIN6157625

Product Details anti-Cyclin A Antibody (Biotin)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human, Mouse, Cow, Monkey, Green Monkey, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-CyclinA2 (E67), Biotin Conjugate

Characteristics: This antibody recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Full length bovine Cyclin A protein

Clone: E67

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

K-562 and HeLa Cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: CyclinA2 (CCNA2 Products)

Synonyms: CCN1 antibody, CCNA antibody, AA408589 antibody, Ccn-1 antibody, Ccn1 antibody, Ccna antibody, CycA2 antibody, Cyca antibody, cb671 antibody, chunp6924 antibody, wu:fi36f03 antibody, CYCA antibody, Cyclin-A2 antibody, ccna2 antibody, ccn1 antibody, ccna antibody, ccna1 antibody, Cyclin-A antibody, cyclinA antibody, cyclin A2 antibody, cyclin A2 S homeolog antibody, cyclin A2 L homeolog antibody, CCNA2 antibody, Ccna2 antibody, ccna2 antibody, ccna2.S antibody, ccna2.L antibody

Background: CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

Molecular Weight: 54 kDa

Gene ID: 890, 58974

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA