Cyclin B1 antibody (CF®647)
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- Target See all Cyclin B1 (CCNB1) Antibodies
- Cyclin B1 (CCNB1)
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Reactivity
- Human, Mouse, Hamster
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Cyclin B1 antibody is conjugated to CF®647
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)
- Purpose
- Mouse Monoclonal anti-CyclinB1 (V92.1), CF647 Conjugate
- Characteristics
- This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Hamster Cyclin B1 protein
- Clone
- V92-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CCNB1 Primary Antibody
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- Application Notes
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunoprecipitation 1-2 μg/500 μg protein, precipitates active CDK1/cyclin B1 complexes
- Optimal dilution for a specific application should be determined by user
- Comment
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Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Cyclin B1 (CCNB1)
- Alternative Name
- CyclinB1 (CCNB1 Products)
- Synonyms
- ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody
- Background
- CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1
- Molecular Weight
- 55-62 kDa
- Gene ID
- 891, 23960
- UniProt
- P14635
- Pathways
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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