HLA DQ antibody (Biotin)
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- Target See all HLA DQ (HLA-DQ) products
- HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))
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Reactivity
- Human, Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HLA DQ antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-MHC-II (HLA-DQ) (SPV-L3), Biotin Conjugate
- Characteristics
- This antibody recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- T4-positive CTL clone HG-38
- Clone
- SPV-L3
- Isotype
- IgG2a kappa
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- Application Notes
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Raji cells. Tonsil or lymph node
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))
- Alternative Name
- MHC-II (HLA-DQ) (HLA-DQ Products)
- Molecular Weight
- 60 kDa
- Gene ID
- 3117, 3118, 3119
- UniProt
- P01908, P01909, P01920
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