beta-2 Microglobulin antibody (CF®568)

Catalog No. ABIN6175186

Product Details anti-beta-2 Microglobulin Antibody (CF®568)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Beta-2 Microglobulin (B2M) (B2M/961), CF568 Conjugate

Characteristics: This antibody recognizes a protein of 12 kDa, identified as β2-microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an i± heavy chain that contains three subdomains (i±1, i±2, i±3) and a non-covalent associating light chain, known as i²-2-Microglobulin. i²-2-Microglobulin associates with the i±3 subdomain of the i± heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The i±1 and i±2 domains of the i± heavy chain form the peptide antigen-binding cleft. Mutations in the i²-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Full length recombinant human B2M protein

Clone: B2M-961

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: Beta-2 Microglobulin (B2M) (B2M Products)

Synonyms: Ly-m11 antibody, beta2-m antibody, beta2m antibody, bwm antibody, wu:fa94c05 antibody, wu:fb10a09 antibody, b2m-W01 antibody, b2m-W03 antibody, b2m-Z01 antibody, b2m-Z02 antibody, b2m-Z03 antibody, B2M antibody, b2m antibody, B2MG antibody, wu:fb66f11 antibody, wu:fj01f08 antibody, zgc:64161 antibody, b-2-m antibody, beta-2-microglobulin antibody, beta-2 microglobulin antibody, beta-2-microglobulin S homeolog antibody, beta-2-microglobulin, like antibody, B2M antibody, B2m antibody, b2m antibody, b2m.S antibody, b2ml antibody

Background: B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p

Molecular Weight: 12 kDa

Gene ID: 567, 534255

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process