BRCA1 antibody (AA 445-620) (CF®488A)
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- Target See all BRCA1 Antibodies
- BRCA1 (Breast Cancer 1 (BRCA1))
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Binding Specificity
- AA 445-620
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This BRCA1 antibody is conjugated to CF®488A
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-BRCA1 (BRCA1/1398), CF488A Conjugate
- Characteristics
- The BRCA1 gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)
- Clone
- BRCA1-1398
- Isotype
- IgG1
- Top Product
- Discover our top product BRCA1 Primary Antibody
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- Application Notes
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Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- BRCA1 (Breast Cancer 1 (BRCA1))
- Alternative Name
- BRCA1 (BRCA1 Products)
- Synonyms
- BRCAI antibody, BRCC1 antibody, BROVCA1 antibody, IRIS antibody, PNCA4 antibody, PPP1R53 antibody, PSCP antibody, RNF53 antibody, iris antibody, pscp antibody, brcai antibody, brcc1 antibody, rnf53 antibody, brca1 antibody, BRCA1 antibody, LOC100224649 antibody, BRCA1, DNA repair associated antibody, breast cancer 1 antibody, breast cancer 1, early onset antibody, breast cancer 1 L homeolog antibody, BRCA1 antibody, brca1 antibody, LOC755086 antibody, Brca1 antibody, brca1.L antibody
- Molecular Weight
- 220 kDa
- Gene ID
- 672, 194143
- UniProt
- P38398
- Pathways
- Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
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