BRCA1 antibody (AA 445-620)

Catalog No. ABIN6175550

Product Details anti-BRCA1 Antibody

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Binding Specificity: AA 445-620

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Purpose: Mouse Monoclonal anti-BRCA1 (BRCA1/1472), Purified, with BSA

Characteristics: BRCA1 is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and also acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). It associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. It plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in BRCA1 are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)

Clone: BRCA1-1472

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence 1-2 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Comment:

A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 (BRCA1 Products)

Synonyms: BRCAI antibody, BRCC1 antibody, BROVCA1 antibody, IRIS antibody, PNCA4 antibody, PPP1R53 antibody, PSCP antibody, RNF53 antibody, iris antibody, pscp antibody, brcai antibody, brcc1 antibody, rnf53 antibody, brca1 antibody, BRCA1 antibody, LOC100224649 antibody, BRCA1, DNA repair associated antibody, breast cancer 1 antibody, breast cancer 1, early onset antibody, breast cancer 1 L homeolog antibody, BRCA1 antibody, brca1 antibody, LOC755086 antibody, Brca1 antibody, brca1.L antibody

Molecular Weight: 220 kDa

Gene ID: 672, 194143

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus