BCL6 antibody (AA 256-389) (CF®594)
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- Target See all BCL6 Antibodies
- BCL6 (B-Cell CLL/lymphoma 6 (BCL6))
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Binding Specificity
- AA 256-389
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This BCL6 antibody is conjugated to CF®594
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-Bcl-6 (BCL6/1526), CF594 Conjugate
- Characteristics
- This antibody recognizes a protein of 95 kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( L&H ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human bcl-6 protein fragment (aa256-389) (exact sequence is proprietary)
- Clone
- BCL6-1526
- Isotype
- IgG1
- Top Product
- Discover our top product BCL6 Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Raji or Ramos cells. Tonsil or Hodgkin s lymphoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- BCL6 (B-Cell CLL/lymphoma 6 (BCL6))
- Alternative Name
- Bcl-6 (BCL6 Products)
- Synonyms
- bcl5 antibody, laz3 antibody, bcl6a antibody, znf51 antibody, zbtb27 antibody, MGC132260 antibody, Bcl5 antibody, BCL5 antibody, BCL6A antibody, LAZ3 antibody, ZBTB27 antibody, ZNF51 antibody, B-cell CLL/lymphoma 6 L homeolog antibody, B-cell CLL/lymphoma 6 antibody, B cell leukemia/lymphoma 6 antibody, bcl6.L antibody, bcl6 antibody, BCL6 antibody, Bcl6 antibody
- Molecular Weight
- 95 kDa
- Gene ID
- 604, 478588
- UniProt
- P41182
- Pathways
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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