c-MYC antibody (C-Term) (CF®405S)

Catalog No. ABIN6175742

This anti-c-MYC antibody is a Mouse Monoclonal antibody detecting c-MYC in IF and FACS. Suitable for Human, Chimpanzee and Gorilla.

Quick Overview for c-MYC antibody (C-Term) (CF®405S) (ABIN6175742)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human, Chimpanzee, Gorilla

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is conjugated to CF®405S

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CT14-G4

Product Details anti-c-MYC Antibody (CF®405S)

Binding Specificity: AA 408-439, C-Term

Purpose: Mouse Monoclonal anti-c-Myc (CT14.G4), CF405S Conjugate

Characteristics: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQL-RNSCA) from C-terminus of human c-myc, coupled to KLH

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Does not react with mouse, others not known
• Optimal dilution for a specific application should be determined by user

Comment:

HL-60 cells or breast carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: c-Myc

Molecular Weight: 62-64 kDa

Gene ID: 4609, 202453

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect