SOX10 antibody (AA 115-269)
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- Target See all SOX10 Antibodies
- SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))
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Binding Specificity
- AA 115-269
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SOX10 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-SOX10 (SOX10/992), Purified, with BSA
- Characteristics
- This antibody recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Recombinant fragment (aa 115-269) of human SOX10 protein with hexa-histidine tag
- Clone
- SOX10-992
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product SOX10 Primary Antibody
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- Application Notes
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Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HepG2 cells. Melanomas, breast carcinomas, gliomas.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))
- Alternative Name
- SOX10 (SOX10 Products)
- Synonyms
- DOM antibody, PCWH antibody, WS2E antibody, WS4 antibody, WS4C antibody, SOX-10 antibody, SOX10 antibody, dom antibody, ws4 antibody, Dom antibody, Sox21 antibody, SOX9 antibody, cls/sox10 antibody, zgc:100757 antibody, SRY-box 10 antibody, SRY box 10 antibody, SRY-box 10 L homeolog antibody, SRY (sex determining region Y)-box 10 antibody, SOX10 antibody, Sox10 antibody, sox10.L antibody, sox10 antibody
- Background
- DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E (WS2E), WS4, Waardenburg syndrome type 4C (WS4C)
- Molecular Weight
- 49-58 kDa
- Gene ID
- 6663, 376984
- UniProt
- P56693
- Pathways
- Chromatin Binding
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