Melanoma gp100 antibody (CF®647)
-
- Target See all Melanoma gp100 (PMEL) Antibodies
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Melanoma gp100 antibody is conjugated to CF®647
-
Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Mouse Monoclonal anti-Pmel17 / gp100 / SILV (PMEL/783), CF647 Conjugate
- Characteristics
- Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human SILV protein
- Clone
- PMEL-783
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PMEL Primary Antibody
-
-
- Application Notes
-
Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
-
SK-MEL-28 cells or Melanoma
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
-
- Target
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Alternative Name
- Pmel17 / gp100 / SILV (PMEL Products)
- Synonyms
- D12S53E antibody, ME20 antibody, ME20-M antibody, ME20M antibody, P1 antibody, P100 antibody, PMEL17 antibody, SI antibody, SIL antibody, SILV antibody, gp100 antibody, D10H12S53E antibody, D12S53Eh antibody, Pmel17 antibody, Si antibody, Silv antibody, gp87 antibody, RPE1 antibody, MMP115 antibody, silverb antibody, silver antibody, cb397 antibody, fdv antibody, pmel17 antibody, sb:cb397 antibody, silva antibody, wu:fc11g11 antibody, wu:fj24g11 antibody, zgc:136622 antibody, premelanosome protein antibody, premelanosome protein b antibody, premelanosome protein a antibody, PMEL antibody, Pmel antibody, pmelb antibody, pmela antibody
- Molecular Weight
- 90-100 kDa
- Gene ID
- 6490, 95972
- UniProt
- P40967
-