FOXA1 antibody (AA 372-472)
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- Target See all FOXA1 Antibodies
- FOXA1 (Forkhead Box A1 (FOXA1))
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Binding Specificity
- AA 372-472
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FOXA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Specificity
- Does not react with rat
- Purification
- Protein G affinity chromatography
- Immunogen
- A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
- Clone
- FOXA1-1512
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product FOXA1 Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/10^6 cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT
Optimal dilution of the FOXA1 antibody should be determined by the researcher. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- FOXA1 (Forkhead Box A1 (FOXA1))
- Alternative Name
- FOXA1 (FOXA1 Products)
- Synonyms
- HNF3A antibody, TCF3A antibody, Hnf-3a antibody, Hnf3a antibody, Tcf-3a antibody, Tcf3a antibody, fkd7 antibody, forkhead-7 antibody, HNF3-alpha antibody, HNF3alpha antibody, fkh2 antibody, foxa1 antibody, hnf3a antibody, tFoxA1 antibody, tcf3a antibody, xfkh2 antibody, fkh antibody, FOXA1 antibody, Ci-fkh antibody, HNF-3a antibody, forkhead box A1 antibody, forkhead box A1 S homeolog antibody, forkhead homolog antibody, forkhead box A1 L homeolog antibody, FOXA1 antibody, Foxa1 antibody, foxa1 antibody, foxa1.S antibody, fkh antibody, foxa1.L antibody
- Background
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FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
FOXA1 in breast cancer is highly correlated with ERalpha+, GATA3+, and PR+ protein expression as well as endocrine signaling. FOXA1 acts as a pioneer factor for ERa in ERalpha+ breast cancer, and its expression might identify ERalpha+ cancers that undergo rapid reprogramming of ERa signaling that is associated with poor outcomes and treatment resistance. Conversely, in ERalpha−, breast cancer FOXA1 is highly correlated with low-grade morphology and improved disease free survival. FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERalpha−, cancers may identify a subset of tumors that is responsive to other endocrine therapies such as androgen receptor antagonist treatment. [Wiki]
- Gene ID
- 3169
- UniProt
- P55317
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis
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