FOXA1 antibody (AA 372-472)

Catalog No. ABIN6251997

Product Details anti-FOXA1 Antibody

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Binding Specificity: AA 372-472

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: Does not react with rat

Purification: Protein G affinity chromatography

Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.

Clone: FOXA1-1512

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

Optimal dilution of the FOXA1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: FOXA1 (FOXA1 Products)

Synonyms: HNF3A antibody, TCF3A antibody, Hnf-3a antibody, Hnf3a antibody, Tcf-3a antibody, Tcf3a antibody, fkd7 antibody, forkhead-7 antibody, HNF3-alpha antibody, HNF3alpha antibody, fkh2 antibody, foxa1 antibody, hnf3a antibody, tFoxA1 antibody, tcf3a antibody, xfkh2 antibody, fkh antibody, FOXA1 antibody, Ci-fkh antibody, HNF-3a antibody, forkhead box A1 antibody, forkhead box A1 S homeolog antibody, forkhead homolog antibody, forkhead box A1 L homeolog antibody, FOXA1 antibody, Foxa1 antibody, foxa1 antibody, foxa1.S antibody, fkh antibody, foxa1.L antibody

Background: FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

FOXA1 in breast cancer is highly correlated with ERalpha+, GATA3+, and PR+ protein expression as well as endocrine signaling. FOXA1 acts as a pioneer factor for ERa in ERalpha+ breast cancer, and its expression might identify ERalpha+ cancers that undergo rapid reprogramming of ERa signaling that is associated with poor outcomes and treatment resistance. Conversely, in ERalpha−, breast cancer FOXA1 is highly correlated with low-grade morphology and improved disease free survival. FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERalpha−, cancers may identify a subset of tumors that is responsive to other endocrine therapies such as androgen receptor antagonist treatment. [Wiki]

Gene ID: 3169

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis