Calponin antibody

Catalog No. ABIN6252460

Product Details anti-Calponin Antibody

Target: Calponin

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calponin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Calponin antibody.

Clone: CLPN5-1

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Calponin

Target: Calponin

Abstract: Calponin Products

Synonyms: CalPoNin antibody, cpn-1 antibody, cpn-2 antibody

Background: Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Gene ID: 1264

UniProt: P51911