SUMO1 antibody
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- Target See all SUMO1 Antibodies
- SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SUMO1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purification
- Protein G affinity chromatography
- Immunogen
- Recombinant human protein was used as the immunogen for this anti-SUMO-1 antibody.
- Clone
- SPM571
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product SUMO1 Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the anti-SUMO-1 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
- Alternative Name
- SUMO-1 (SUMO1 Products)
- Synonyms
- DAP1 antibody, GMP1 antibody, OFC10 antibody, PIC1 antibody, SENP2 antibody, SMT3 antibody, SMT3C antibody, SMT3H3 antibody, UBL1 antibody, hm:zeh0670 antibody, wu:fb74c02 antibody, wu:fd12c02 antibody, zgc:65934 antibody, zgc:85634 antibody, SUMO1 antibody, dap1 antibody, gmp1 antibody, ofc10 antibody, pic1 antibody, senp2 antibody, smt3 antibody, smt3c antibody, sumo-1 antibody, sumo1 antibody, ubl1 antibody, xsumo-1 antibody, xsumo1 antibody, ARABIDOPSIS THALIANA SMALL UBIQUITIN-LIKE MODIFIER 1 antibody, ATSUMO1 antibody, F10M23.180 antibody, F10M23_180 antibody, SMALL UBIQUITIN-LIKE MODIFIER 1 antibody, SUM1 antibody, SUMO 1 antibody, small ubiquitin-like modifier 1 antibody, pigSUMO1 antibody, SENTRIN antibody, SMTP3 antibody, SUMO-1 antibody, Smt3C antibody, Ubl1 antibody, small ubiquitin-like modifier 1 antibody, small ubiquitin-like modifier 1 S homeolog antibody, small ubiquitin-related protein 1 antibody, SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) antibody, SMT3 suppressor of mif two 3 homolog 1 (yeast) antibody, small ubiquitin-like modifier 1 L homeolog antibody, Sumo1 antibody, SUMO1 antibody, sumo1 antibody, sumo1.S antibody, sumo-1 antibody, sumo1.L antibody
- Background
- This mAb is specific to SUMO-1 and shows no cross-reaction with either SUMO2 or SUMO3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, SUMO2 and SUMO3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized is precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO1 protein localizes to the nuclear membrane.
- Gene ID
- 7341
- UniProt
- P63165
- Pathways
- M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway
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