SLC25A21 antibody

Catalog No. ABIN6252824

Product Details anti-SLC25A21 Antibody

Target: SLC25A21 (Slc25a21) (Solute Carrier Family 25 (Mitochondrial Oxodicarboxylate Carrier), Member 21 (Slc25a21))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC25A21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human ODC-1 protein was used as the immunogen for this antibody.

Clone: ODC1-485

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for SLC25A21

Target: SLC25A21 (Slc25a21) (Solute Carrier Family 25 (Mitochondrial Oxodicarboxylate Carrier), Member 21 (Slc25a21))

Alternative Name: ODC-1 (Slc25a21 Products)

Synonyms: zgc:153387 antibody, ODC antibody, ODC1 antibody, Odc1 antibody, 9930033G19Rik antibody, A630030I10 antibody, BB158148 antibody, solute carrier family 25 (mitochondrial oxoadipate carrier), member 21 antibody, solute carrier family 25 (mitochondrial oxoadipate carrier), member 21 L homeolog antibody, solute carrier family 25 member 21 antibody, solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 21 antibody, slc25a21 antibody, slc25a21.L antibody, SLC25A21 antibody, Slc25a21 antibody

Background: This antibody recognizes a 53  kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Gene ID: 4953

UniProt: P11926

Pathways: SARS-CoV-2 Protein Interactome