Prostate Specific Antigen antibody
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- Target See all Prostate Specific Antigen (PSA) Antibodies
- Prostate Specific Antigen (PSA)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Prostate Specific Antigen antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purification
- Protein G purified
- Immunogen
- Full length human recombinant protein was used as the immunogen for this PSA antibody.
- Clone
- KLK3-801
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PSA Primary Antibody
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- Application Notes
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ELISA: For coating use Ab at 1-5 μg/mL, order Ab without BSA
Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- Prostate Specific Antigen (PSA)
- Alternative Name
- PSA (PSA Products)
- Synonyms
- APS antibody, KLK2A1 antibody, PSA antibody, hK3 antibody, KLK3 antibody, Klk1c10l2 antibody, RSGK-50 antibody, RSKG-50 antibody, rGK-3 antibody, Kal-3 antibody, Kal3 antibody, Klk3 antibody, AAP-S antibody, MP100 antibody, Psa antibody, R74825 antibody, goku antibody, kallikrein related peptidase 3 antibody, kallikrein 3 antibody, kallikrein 1-related peptidase C3 antibody, kallikrein B, plasma 1 antibody, aminopeptidase puromycin sensitive antibody, KLK3 antibody, Klk1c3 antibody, Klkb1 antibody, Npepps antibody
- Background
- Prostate specific antigen / PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. Using a Prostate specific antigen antibody, the protein can be detected in the sera of patients with prostatic carcinoma and expression level correlates positively with the likelyhood of cancer.
- Gene ID
- 354
- UniProt
- P07288
- Pathways
- Complement System
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