Tyrosinase-Related Protein 1 antibody

Catalog No. ABIN6252894

Product Details anti-Tyrosinase-Related Protein 1 Antibody

Target: Tyrosinase-Related Protein 1 (TYRP1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tyrosinase-Related Protein 1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the TRP1 antibody.

Clone: SPM611

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the TRP1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: TRP1 (TYRP1 Products)

Synonyms: CAS2 antibody, CATB antibody, GP75 antibody, OCA3 antibody, TRP antibody, TRP1 antibody, TYRP antibody, b-PROTEIN antibody, LOC397853 antibody, Trypsin antibody, hm:zeh0659 antibody, tyrp1 antibody, zgc:100893 antibody, B antibody, TYRP1 antibody, tyrp-1 antibody, TRP-1 antibody, Tyrp antibody, b antibody, brown antibody, isa antibody, tyrosinase related protein 1 antibody, protease, serine 1 L homeolog antibody, tyrosinase-related protein 1b antibody, tyrosinase-related protein 1 antibody, tyrosinase-related protein 1 L homeolog antibody, tyrosinase-related protein 1a antibody, TYRP1 antibody, prss1.L antibody, tyrp1b antibody, Tyrp1 antibody, tyrp1.L antibody, tyrp1 antibody, LOC100136490 antibody, tyrp-1 antibody, tyrp1a antibody

Background: It reacts with a 75  kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Gene ID: 7306

UniProt: P17643