IL-6 antibody (Internal Region)

Catalog No. ABIN6262529

Product Details anti-IL-6 Antibody

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: IL6 Antibody detects endogenous levels of total IL6.

Predicted Reactivity: Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human IL6, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-IL-6 antibody (ABIN6262529)

Yi, Shen, Zhao, Shao, Liu, Cui, Wang, Wang, Guo, Kang, Lv, Xing, Zhang: "Inflammation-mediated SOD-2 upregulation contributes to epithelial-mesenchymal transition and migration of tumor cells in aflatoxin G1-induced lung adenocarcinoma." in: Scientific reports, Vol. 7, Issue 1, pp. 7953, (2018) (PubMed).

Ji, Zheng, Liu, Ren, Gao, Zhang, Li: "Resveratrol promotes oxidative stress to drive DLC1 mediated cellular senescence in cancer cells." in: Experimental cell research, Vol. 370, Issue 2, pp. 292-302, (2018) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6 (IL6 Products)

Synonyms: BSF2 antibody, HGF antibody, HSF antibody, IFNB2 antibody, IL-6 antibody, Il-6 antibody, ILg6 antibody, Ifnb2 antibody, il6 antibody, CHIL-6 antibody, interleukin 6 antibody, interleukin-6 antibody, IL6 antibody, Il6 antibody, il-6 antibody, IL-6 antibody

Background:

Description: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

Gene: IL6

Molecular Weight: 24kDa

Gene ID: 3569

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome